AIM:To investigate whether pretreatment with repeated electroacupuncture (EA) could induce ischemic tolerance against transient spinal cord ischemia in rabbits.METHODS:24male New Zealand white rabbits were randomly assigned to 3 groups(n=8 each),animals in the control group received no treatment;animals in the SP and EA group received sodium pentobarbitone 30mg/kg each day for 5 days;animals in EA group were also received electroacupuncture at the Zusanli acupoint 30min a day for 5days.24hours after the last treatment,spinal cord ischemia was induced by an infrarenal aortic occlusion for 20min.Hind-limb motor function was determined with the Tarlov criteria at 4,8,12,24 and 48h after reperfusion.All animals were sacrificed at 48h after reperfusion and the spinal cords(I5) were remoed immediately for histopathologic study.RESULTS:The neurologic outcome and histopathology(48h) in the EA group were significantly better than the control group(P=0.006).CONCLUSION:Pre-ischemic treatment with electroacupuncture significantly reduces spinal cord ischemia-reperfusion injury in rabbits.
Due to partial or systematic reasons,long term teeth deficiency can break the dynamic balance of osteogenesis and osteo-destruction.The dental alveolar ridge may be absorbed and atrophied to a low,narrow and notched shape,just like an edge.as a result,the attachment plate between the base and the dental alveolar ridge is decreased and can affact retention and stability of the denture.It is difficult to achieve the goal of mastication function rehabilitation.So to augment dental alveolar ridge by surgical operation is one of the most important tasks of denture preposthetic surgery.We thus review an assay about guided bone regeneration(GBR) in surgery repair technique.
AIM:To investigate the osseointegration process of titanium implant and non-vascularized iliac bone grafts.METHODS:12 mongrel were divided into 4 groups randomly.Bone grafts were resected from iliac crest and then transplanted to the other side.Animals were skilled in different time after surgery,X-ray pictures were taken,then histological observation were done.RESULTS:At 3rd week,bone grafts dissolved,resorbed or necrosis partly;At 6th week,new bone began to regenerated;At 9th week,the amount of new bone increased;At 12nd week,bone interface around implant formed,without soft tissue interrupt.CONCLUSION:Osseointegration can formed between non-vascularized iliac bone grafts and titanium implant.
AIM：To evaluate the surgical treatment methods of giant cell tumors(GCT) of long bone in conjunction with inserted microwave antennas induced hyperthermia.METHODS:46patients,included the surgical procedures,the oncology results,the functions of the limbs and the complications were analyzed.RESULTS:Follow-up 3.5 to 9 years(mean 5.5years).All patients were evaluated according to oncological and orthopaedic criteria.Two tumors were recurred.Orthopaedic furction were perfect in 44 patients and were fair in 2.Infection was found in 2 patients.CONCLUSION:The surgical procedure to treat the giant cell tumors of long bone by inserted microwave antennas induced hyperthermia is a definitive surgical method which is safe and confident.
AIM：To investigate the effect of allogeneic acellular dermal matrix(ADM) on cograft in joint functional positions of patients with post-burn plastic operation.METHODS:9 patients with hypertrophic acar and joint dysfunction after severe burns were used.After pre-treating with trypsin and TritonX-100,13 reticulated ADM were overlapped with autogenous ultrathin split-thickness skin grafts(USTS),and were transplanted to the acar excision wounds in the joints of four limbs at the same time.The neighbouring autogenous thin split-thickness skin grafts(TSTS) were used as control.RESULTS:The composite skin grafts as well as the controls were all survived.The rejection and hypertrophic scars were not found during (1-5) years follow-up studies.The apearance,fiber and function of composite skin grafts were near to normal skins.CONCLUSION;The ADM could be used to joint functional positions of patients with post-burn hypertrophicscare and could produce satisfactory plastic results as dermal substitute.
AIM：To study the relationship between the expression of Fas/FasL protein and apoptosis in rats after renal ischemia/reperfusion.METHODS:Establish the models of renal ischemia/reperfusion injury in rat and SABC immunohistochemical methods were used to detect the changes of expression of Fas/FasL protein.Pathomorphological changes in renal ischemia/reparfusion injury were observed.RESULTS:The expression of Fas/FasL proteins was negative in the sham operated group.Fas/FasL proteins were increased in renal in ischemia/reperfusion group,and gradually upregulated with the duration of ischemia or reperfusion and peaked at 72h of reperfusion.The expression of Fas/FasL proteins was stronger in 60min ischemia group than 30min ischemia group and they were mainly expressed in renal tubule.We observed local necrosis and inflammatory cells infiltration around infracted areain ischemia/reperfusion group by HE dyeing methods.And the necrosis area was mainly occurred around proximal convoluted tubule.CONCLUSIONS:These findings suggested that Fas/FasL proteins were over expressed after ischemia/reperfusion injury in rats renal.And Fas/FasL system was involved in the process of renal ischemia/reperfusion injury.
AIM:To study the effects of adrenocorticotropic hormone(ACTH) and electroacupuncture (EA) on formalin-induced nitric oxide synthetase (NOS)-positive neurons increases in the spinal cord or rats.METHODS:ACTH was administered by intrathecal injection (i.t.)and EA stimulation on “jiaji” point was performed by 1 mA 50Hz,5mA 5Hz and 1mA 5Hz respectively.The NOS-positive neurons were assayed by NADPH-diaphorase histochemistry.RESULTS:The results showed that both ACTH(0.5u,i.t.)and EA stimulation (1mA 50Hz,5mA 5Hz,1mA 5Hz) on “jiaji” point 30min significantly reduced the formalin-induced NOS-ositive neurons in the rat dorsal horn.The combinative use of ACTH (0.5u,i.t.) and EA(1mA 5Hz) caused a more marked reduction of the numbers of NOS positive neurons than that of the single ACTH or EA.Those effects were partially reversed by pretreatment with either the substrate of NOS,L-arginine (10nmol,i.t.)or opioid antagonist naloxone(10g,i.t.).CONCLUSION:These results suggests that both ACTH and EA might inhibit the formalin-induced NOS-positive neurons increases and have a synergic effect acting via a different pathway.
AIM:To eluciate the role of neuron specific enolase(NSE) in predicting prognosis after severe head injury.METHODS:30 patients with severe head injury were involved into this study,ranging from 26 to 64 years old.Concentration of NSE in serum was measured in all cases within 12 hours after head injury.And prognosis of all patients were evaluated by GOS.RESULTS:The concentration of NSE in serum of both groups,with good or poor outcome,were higher than normal group.The concentrations within 12 hours after head injuries had a cose relationship with the prognosis.As a serum marker to assess the prognosis,NSE had high positive prediction ratio.CONCLUSION:The finding suggested that NSE may be a promising predictor for assessing the prognosis after severe head injury.
AIM：To illustrate the magnetic resonce(MR) myelography features in the disease of lumbosacral nerve roots.METHODS:MR myelography using heavily T2 weighted spin-echo imaging with fat supression was performed in 75 cases with chronic pain of waist on a Siemens Magneton Impect 1.0 TMR unit.Maximum intensity projection(MIP) was used for image reconstruction.RESULTS:The thecal maugins,nerve roots and nerve root sheaths were well demonstrated on MR myelography image.In 75 patients with chronic of waist,nerve root disease was found in 11 cases.6nerve root abnormality (8%),2perineural cyst(3%),3 neurofibroma(4%) had their own MR myelography features.CONCLUSION:MR myelography can show the morphologic and structural change of lumbosacral nerve roots.In MR myelography image,disease of lumbosacral nerve roots has a characteristic finding.MR myelography in very useful in diagnosis of nerve-root disease.
AIM：To investigate the effects of preoperative other diseases on the complications of senile cerical vertebral operation in perioperative period.METHODS:800 senile cervical vertebral cases were divided into two group:group A included the cases without other comorbidities,and group B included the cases with hypertension,diabetes mellitus,coronary heart disease (GHD) or chronic obstructive pulmonary disease(COPD).We retrospectively analyzed the changes of BP,oxygen saturation (SaO2) and the incidence of cardiac arrhythmia during operation of group A and B.RESULTS:In perioperative period,the incidence of B reduction or rising and cardiac arrhythmia in group B was more than that of group A.Incidence of SaO2 decreasing increases apparently in each stage and incidence of cerebrovascular,pulmonary complications and asphyxia led by incision bleeding increases apparently.CONCLUSION:The hypertension,CHD,diabetes mellitus and COPD were the main reasons of complications of the heart,the cerebral vessels and the lung,kidney and the incision bleeding in perioperative period of senile patients.
AIM：To provide a new way of operation for repairing fracture necrosis of proximal humerrus.METHODS:Observing the course,branches and distributions of thoracoacromia artery on 40 sides of abult cadaveric.RESULTS:(1)Sending off 3 to 5 periosteal branches with diameter of 0.3 to 0.5mm along the acromial branch of thoracoacromial,which distributed at the middle outboard and formed the part of acromial artery net.The acromial length was (5.1±0.5)cm with diameter of (1.2±0.2)mm.(2)Sending off 1 to 3 periosteal branches with diameter of (0.2 to 0.5)mm from the clavicular branches of thoracoactomial artery,which distributes at the inside part one second.The length was (2.0±0.1)cm with diameter of (1.2±0.2)mm.CONCLUSION:A new operation can be designed for repairing fracture necrosis of proximal humerus by transposing clavicle periosteal(bone) flap with the acromial branch of toracoacromial artery.
AIM:To observe the clinical effects of three-dimensional instrumentation in the correction of frontal,sagittal and axial plane deformity of scoliosis.METHODS:From June 1996 to June 2001,52 cases of scoliosis were corrected with three-dimensional instrumentation.Among them,36cases were treated with CD system,5 cases were treated with TSRH,6 cases were treated with China Greatwall and 5 cases were treated with Scofix.RESULTS:All the patients were followed-up for an average of 2 years(ranging from 1 to 4 years).The averaged frontal curve was 25.5°（ranging from 10° to 46°),the average correction rate was 63.6%.The average sagittal curve was 27°(ranging from 20° to 35°).CONCLUSION:Three-dimensional instrumentation is effective in three-dimensional correction and is an excellent tool for scoliosis correction.The fixation isreliable and the complications rarely occurred.
AIM：To analyze the effect of three kinds of method on different types of Pilon fracture and advance the beat treatment plan.METHODS:From March 1989 to August 2000,107 patients were regarded as having Pilon fracture by two hospitals,among which 76 cases were followed up.They were divided into three groups according to treatment method.A group included 24 cases with the treatment of manual reduction,traction of calcaneus and plaster exopexy.B group included 30 cases with the treatment of AO key-shaped anatomical steal plate.C group included 22 cases with the treatment of limited internal fixation combined with exopexy stand.RESULTS:After 6months to 8years follow-up,it was found that three kinds of treatment methods had obvious differences on the complications and effects of different types of fracture.In A group,the excellent and good rate of treatment on I type fracture was 70%,Ⅱ type was 25%,Ⅲ type was 0%.In B group,the excellent and good rate of treatment on I type fracture was 78.6%,Ⅱ type s 87.5%,Ⅲ type was 37.5%.In C group,the excellent and good rate of treatment on I type was 75%,Ⅱ type was 80%.CONCLUSION:Summarizing kinds of factors,the treatment of A group applied in I type fracture,B group applied in Ⅱ type fracture and C group applied in Ⅲ type fracture.
AIM：To investigate the effect of proinflammatory cytokine and anti-infalammatation cytokine on liver and lung tissues in rats with endotoxemia.METHODS:Male Wistar rats were randomly divided into 4 groups:group treated with stimulating vagus nerve,group receiving lipopolysaccharide (LPS) intravenous injection after transecting vagus nerve,group treated with sham operation and group treated with injecting LPS intravenously alone,and then measured the levels of TNF-α in liver and lung and those of cortisol and Alanine aminotransferase(ALT) in plasma.RESULTS:Compared with group treated with sham operation,LPS-treated groups showed a significant increase in TNF level,which was at most 15 fold higher than that of the former group.There was a significant decrease in TNF level in group treated with stimulating vagus nerve,compared with both group receiving LPS intravenous injection after transecting vagous nerve and group treated only with LPS.In addition,we observed plasma cortisol level in LPS-treated group was much higher than other 3 groups and the plasma ALT level was greatly lower than that of group treated only with LPS.CONCLUSION:Stimulating vagous nerve can significantly decrease the production of proinflammatory cytokine and alleviate inflammation in rats with endotoxemia.
To investigate the maitainance effect of enteral feeding ELENTAL on inteatine,liver and nutritional status of bumed patients.METHODS:30 cases of burned patients were randomly devided into ELENTAL feeding group(Egroup) and intravenous nutrition group(control,D).The whole calorie in group E were supplied by feeding elemental dist ELENTAL and those in group D were supplied by intravenous nutrition from the third day after burning.the supplemented calorie and nitrogen were equal in two groups.Before the ezperiment and on the 5th day and 10th day,blood were drawn to examine the levels of and serum transferring.RESULTS:There were no significant diference of serum lipid and serum transferring levels on the 7th day and 10th day in burned patients.CONCLUSION:ELENTAL diet can protect intestine effectively and improve nutritius status of the burned patients.
AIM：To investigate the hemostasis effect of hemostasis sponge of collagen and chitosan compound invitro and in vivo.METHODS:The hemostasis sponge of collagen and chitosan compound was made by mixing collegen I extracted by enzymelytic methods and crosslinked by glutaric dialdeiyde and then lyophilized.The New zealand rabbit were created with a 1cm×1cm wound in the center artery of the ear.The center artery of the ear were transected and record the hemostasis time since pressured with hemostasis sponge of collagen and chitosan compound and obsevve its effects.In other experiements,1cm×1cm bleeding wound were established in the liver of the rabbit.Then pressured with hemostasis sponge of collagen and chitosan compound on the wounds and record the hemostasis time;The four corner of the hemostasis sponge of collagen and chitosan compound were sutured to investigate the absorption situation with the control of gelatin treated with same procedures.RESULTS:The bleeding time using hemostasis sponge of collagen and chitosan compound was 120 seconds compared with 600 seconds of using gelatin,and the hemostasis time using hemostasis sponge of collagen and chitosan compound was 40 weconds compared with 75 seconds of using gelatin.Both the hemostasis sponge of collagen and chitosan compound and gelatin can be degradated within 5 months.CONCLUSION:Hemostasis effect of hemostasis sponge of collagen and chitosan compound is better than that of gelatin and it has an promising future in surgery.
AIM:To investigate effects of heart automatic nerve variations and hypothermia(HT) therapy on heart rate variation(HRV).METHODS:We studied the dynamic electrocardiagraph(ECG) of 41 cases of severe brain injury.The patients were devided into HT therapy group(n=21,body temprature 32-33℃),non-HT therapy group(n=20) and normal control(n=20).RESULTS:In group 1,the reduction of time domain indexes and frequency domain indexes were 3-6 folds and 30-50folds respectively compared with those of the control group,while the corresponding indexes reduction were 2-3 folds and 4-5folds in the group 2(P<0.05,P<0.01,respectively).The difference between groups were significant(P<0.05-0.01);the HRV reduction was more significant in the cerebrovascular accident patients than that of brain injured(P<0.05-0.01);the HRV indexes increased significantly in resuscitated patients than those in coma(P<0.01);the HRV in dead was much lower than that in survival(P=0.01).CONCLUSION:Injury of central nervous system can induce abnormal function of automatic nerve of heart and reduction of HRV.HT therapy can reduce HRV on a higher level;HRV examination can be applied into the prediction of prognosis of the brain injured.
AIM:To investigate the antimicrobial spectrum of nanometer materials to 33 strains of ordinary anaerobic pathogenic bacteria isolated from 11 genera.METHODS:The anti-anaerobic effects of nanometer materials were examined and measured by test tube dilution method.RESULTS:In most tubes,there were no bacteria growth in nanometer suspension.CONCLUSION:there is a wide antimicrobial spectrum of nanometer materials and it can effectively inhibit the growth of the anaerobic bacteris.
AIM:To investigate the effects of selective posterior rhizotomy(SPR)to spastic cerebral palsy.METHODS:55 patients with spastic cerebral palsy who were treated with SPR between April 1999 and January 2002 were followed up and compared their changes of muscle force,muscle tone,the limb function preoperatively and postoperatively.RESULTS:51 had selective lumbosacral posterior rhizotomy.In 35 cases who could not walk preoperatively,25 could walk alone.6 cases imroved their gait ad walk ability and 4 did not significantly.20 cases who walked claudicantly preoperatively,can walk nimbly and firmly.Their step width extended and gait improved apparently.4 cases had selective cervical posterior rhizotomy.They had their spasm of upper limbs relieved,joint function improved,and clawhand disappeared.In this group we didn‘t find severe complications.CONCLUSION:Selective posterior rhizotomy has curative effect to spastic cerebral palsy and has no severe complications.Its long-term effects still need observations.