AIM：To investigate the expression of Fos and Jun proteins in rat brains after focal cerebral ischemia followed by reperfusion and effects of RSM and astragus,METHODS:30 SD adult male rats were divided into 5 groups at random.Group A:sham-operated group;Group B:model group;Group C:treated with RSD;Group D: treated with astragus;Group E:treated with RSM and astragus,The immunohistochemistry and medical image processing system(MIPS) were used to measure the numbers and mean grey levels of Fos and Jun protein positive cells in rat cerebral cortex of 5 groups.RESULTS:(1)In cerebral cortex of group B,C,D,E,the numbers of Fos and Jun positive cells were more than those in group A and mean grey levels of Fos and Jun positive cells were lower than those in group A(P<0.01);(2)In cerebral cortex of ischemic sides in group C,D,E,the numbers of Fos and Jun positive cells were less than those in group B and mean grey levels of Fos and Jun positive cells were higher than those in group B(P<0.01);(3)Group E had more significant effects than group Cor group D(P<0.01).CONCLUSION:The expression of Fos protein and Jun protein in model group increased significantly ,compared with sham-operated group;RSM,astragus,RSM and astragus all could inhibit partly the expression of Fos protein and Jun protein after cerebral ischemia and reperfusion;Prescription of RSM and astragus had stronger inhibiting effects than RSM or astragus,It may be one of mechanisms that ischemic stoke is treated by reinforcing Qi and activating blood circulation therapy in TCM clinic.
AIM:To disdcover the level of estrogen(ES) and nitro oxide(NO) in postmenopausal women with myocardial infarction(MI) and their relation.METHODS:Using the technique of radio immunoassay and cop-per-cadmium-plating reducing method to measure the blood level of E2 and NO of 30 postmenopausal women with MI and 30 healthy postmenopausal women,RESULTS:The blood level of E2 and NO of postemenopausal women with MI was significantly decreased compared with control group (100±40)pmol/L vs (158±11）pmol/L(P<0.01)(50±30）μmo/L vs(70±40)μmol/L,there was no relation between E2 and NO in postmenopausal women with MI and there in no relation between E2 and NO.
目的：分析脑卒中偏瘫患者治疗前后影响日常生活活动能力（activities of daily living,ADL）的相关因素，方法：对53例脑卒中偏瘫患者治疗前后进行上下肢功能，ADL等相关因素评定。结果：影响入院前ADL（ADL0）的正性因素包括肌张力、治疗前下肢运动能力，负性因素为年龄，尿便失禁，入院前居住地及发病次数。治疗后ADL（ADL1）的正性因素有ADL0和病变性质，而患肢腱反射的亢进或减弱和发病后住院时间则是ADL1预后的负性因素。家庭人数与ADL1恢复程度呈负相关（r=-0.527,n=53）。结论：肌张力和患肢腱反射是影响患者ADL恢复的有利因素；家庭人数多是ADL恢复的阻碍因素，提示对家属进行康复意识的教育非常必要。
目的：通过各种MRI扫描序列比较，选择颞下颌关节紊乱病（temporomandibular disorder,TMD)的最佳检查方法。方法：对13例患者采用PHILIPS公司的Gyroscan Intera型超导磁共振成像系统。磁场强度1．5T，选择11cm双侧表面线圈，9例采用双侧对比成像，4例单侧成像，共22例，扫描闭口矢状位的自旋回波（SE）T1WI，快速自旋回波（FSE）T2WI，质子密度（PDW）及各种快速梯度回波（Fast Field Echo,FFE)T1W和T2W序列；冠状位扫描T1W和张口矢状位T1W。结果：各种扫描序列均可以显示颞下颌关节（TMJ）的基本解剖结构，从对比度、空间分辨率、信噪比和扫描时间几方面评价，FFE序列在缩短扫描时间的同时保持了一定的信噪比，优于常规SE序列，3D／FFE序列分辨率高，对关节盘、软骨显示好，并能在任意方向多平面重建，可进行精确的体积测量，结论：FFE序列优于常规SE序列，3D／FFE序列更适合于TMJ的检查。
To investigate the sleep quality in elderly patients with obstructive sleep apnea syndrome (OSAS),METHODS:54 cases of elderly patients with OSAS were evaluated by the pittsburgh sleep quality index (PSQI) questionnaire,RESULTS:Among 54 OSAS patients 35 cases (65%) reported poor quality of sleep,only 7(13%) cases reported good.A significant negative correlation was found between apnea/hypopnea index (AHI) and PSQI,and part of its components .CONCLUSION:Elderly patients with OSAS ofter complain of poor quality of sleep.Daytime dysfunction,poor subjective sleep quality,low habitual sleep efficiency and long sleep latency constitutes the principal parts of the symptoms about sleep.
AIM：To study influence of Valeriana officinalis var.latifolia(VOL) on expression of C-Fos,C-Jun after focal cerebral ischemis,METHODS: Inducing rat model of reversible middle cerebral artery occlusion(MCAO) using Koizumi‘s intraluminal suture occlusion method.48 male rats were divided into 5 groups randomly,pseudo-operation group,MCAO group,saline control group,VOL group.2 hours after MCAO,we took gastric gavage with VOL and saline,8 hour per time,and took out of brain to test C-Fos,C-Jun expression immunohistochemically at the 5th day after operation,RESULTS:There was no positive cell in each hippocampus zone of normal group;we observed C-Fos,C-Jun positive cells in each Hippocampus zone after MCAO;Density of C-Fos,C-Jun positive cells of VOL group were apparently lower than that of simple ischemia group.CONCLUSION:VOL can relieve histopathological lesions after cerebral ischemia and promote protection function of rat through inhibiting the expression of C-Fos,C-Jun expression.
AIM：To investigate whether cocktail theraphy combined with of neuroprotetants may have more advantages over single agents in treating focal cerebral ischemic cascade,METHODS:With the use of suture occlusin techique,the right middle cerebral artery in rats was occluded.Tirty minutes later,Frutose-1,6-diphosphate(FDP)(50mg/kg,n=20),MK-801(1mg/kg,n=20) and N-acetylcystein (NAC)(150mg/kg,n=20) were singly or combinantly infused intraperitoneally,At the same time the cocktail-treated group(n=20) were infused the above agents combinationly and the control group(n=20) were infused normal saline intraperitoneally.6 hours and 24 hours after focal cerebral ischemia the animals were weighted and neurologically assessed on 5-point scale,The animals were killed,brains were stained 2,3,5-triphenyltetrazolium chloride for assessment of the infarct volume and then embedden onto slides with paraffin for morphological assessment and terminal transferase dUTP nick ending labeling (TUNEL) were carried out for apoptosis and immunohistochemistry were carried out to investigate the changes in bcl-2 .RESULTS:All Neuroprotectants decreased volume of infarction(P<0.05,P test).While cocktail-treated group showed more distinct decrease than other groups(P<0.05,F test),FDP-treated group did not decrease the apoptosis of the neurons and did not increase the bcl-2 expression as well MK-801-treated group,NAC-treated group and cocktail-treaed group significantly decreased the apoptosis of the neurons and increased the bcl-2 expression (P<0.05,F test). With cocktail-treated group showing more distinct effect (P<0.05,F test).CONCLUSKON:Cocktail may be more effective than single neuroprotectant in this modle.
AIM:To investigate the magnetic resonance imaging(MRI)findings of lipid storage myopahty(LSM)，evaluate the clinical value of MRI for LSM.and thigh,spin echo sequence,fast spin echo sequence and short tau inversion recovery(STIR)were utilized .RESULTS:The signal intensity of diseased muscle of 8 cases with LSM was hyperintense on T2-weighted images (T2WI) and T1-weighed images (T1WI),and hyperintense signal intensity on T2WI and iso-intense on T1WI was seen on 2 patients as well.CONCLUSION:MRI can provide objective data for clinical diagnosis,therapeutic evaluation and folow-up.It can also help th decide the accurate localiztions for biopsies.
AIM:To investigate the effect of temporomandibular joint osition and remolding process and the signs and symptoms of temporomandibular joint in patients undergoing orthognathic surgery, METHODS:The stadard Schuller‘s position radiographs were used to examine the position temporo-mandibular joint form and condylar position change of 32 case after orthog-nathic surgery,Helkimo index was used to analyze the anamnestic and clin-ical data.RESULTS:Changes of condylar position had happened after the operation,but within one year the conlylar had resumed the same position as the preoperative.The change of temporomandibular joint symptoms and signs atfer orthognathic surgery was not significant .CONCLUSION:The effect of orthognathic surgery on the condylar position and TMJ form is not significant,and most of the changes are within the normal adaptability of temporomandibular joint.
AIM:To clone and identify genes differentially expressed in the EB virus-transformed human B cell.METHODS:Suppression subtractive hybridization was used to construct the library which contains the differentiately expressed cDNAs in EB virus-transformed human B cell .Then the isolated genes were cloned and sequenced and identifed by RT-PCR ,Nucleic acid homology searches were performed using the BLAST program.RESULTS:By this technique,4 differentiately expressed gene cDNA fragments of EB virus-transformed human B cell were obtained .CONCLUSION:SSH is an effective method to isolate differentiately expressed genes.