目的：探索修复面神经缺损的新的有效替代材料。方法：20只大鼠随机分成实验组(化学萃取同种异体胫神经移植组。10只)和对照组(自体新鲜胫神经移植组，10只)。大鼠一侧面神经下颌支缺损6mm的模型，以上述两种移植物桥接修复。术后5个月，HRP逆行示踪观察神经通路，以及用苏木精-伊红染色、Luxol Fast Blue染色和透射电镜等观察神经再生的组织学情况。结果：两组均在术侧面神经核发现被标记的神经元细胞，移植段内有大量的新生有髓神经纤维和新生血管等。两组结果相似。结论：化学萃取同种异体神经极有可能成为自体神经的替代材料，应用于面神经缺损的修复。
Periosteum transplantation and bone transplantation are common methods in the treatment of segmental bone defect at present with unsatisfied effectiveness.OBJECTIVE: To discuss effectiveness of united transplantation of free periosteum-bone marrow-calcium phosphate(FP-BM-CPC) in the restoration of segmented bone defect.DESIGN: A self-coutrol prospective study was performed.SETTING and PARTICIPANTS: The study was completed in the Department of Orthopaedics, the Affiliated Hospital of Guilin Medical College and Department of Orthopaedics and Traumatology, the First Affiliated Hospital of Guangxi Medical University. Subjects were 15 New Zealand rabbits in either genders and with a body weight between 1.5 to 2.5 kg provided by Laboratort of Experimental Animals. Guangxi Medical University.METHODS: A bone defect model at 10 mm of midpiece in both radiuses of trialal rabbit was established. Trial group: one side of bone defect was restored by the transplantation of FP-BM-CPC: and the other side was taken as control group.MAIN OUTCOME MEASURES: X-ray plain films and histological exantinations for both radiuses affter 1.2. 4.6 and 8-week surgery.RESULTS: Bone defects in trial group were restored after 8-week injury but disunion in control group.CONCLUSION: United transplantation of FP-BM-CPC has good osteogenesis. which hopefully can be used as an effective method in the restoration of segmental bone defects in low-bearing or uon-hearing parts.
BACKGROUND: The descent of intestinal mucous membranous immune barrier will cause the translocation of opportunistic pathogen, which will lead to enterogenous infection that will affect the rehabilitation of patients‘ organisms and relevant functions, but the mechanism is still not well established.OBJECTIVE: To observe the transition of mIgA in peyer patch lymphocytes (PPL) and the conditions of proliferations in vitro of lamina properia lymphocytes(LPL) in intestinal mucous membranous immune tissues after burn,in order to increase mucous membranous immune barriers, to prevent enterogenous infections and to provide a clinical gist in speeding up rehabilitations in burn patients.DESIGN: A random control vertical study.SETTING and PARTICIPANTS; This study was completed in the Central Laboratory and Department of Burn Surgery, General Hospital of Chinese People‘s Armed Police Forces. Subjects were 20 SPF mice.INTERVENTION: The mice were randondy grouped into control group (n= 10) and burn group/n = 10). Mice in burn group were burnt with 20% of total body surface area(TBSA) in III degree burn and executed after 3-day of injury together with the mice in control group.MAIN OUTCOME MEASURE; IgA plasmoeytes in intestinal lamina propria(LP) were observed by in vivo imnmnohistochemical staining; IgA on the surface of lymphocytes in PP were counted by flow cytometry; in vitro proliferations of LP lymphocytes were assayed after burn; IL-6 in intestinal tissue was assayed b) ELISA.RESULTS: The positive rate of IgA in PP after 3-day of burn was lower than that of control group: the numbers of IgA ptasmocytes in LP after burn were significantly less than the level before burn, in vitro proliferation of the lymphocytes in LP was distinctly decreased after burn; the level of IL-6 in intestinal tissue had no significant difference compared with that of control group.CONCLUSION: Burn irffdbits the proliferation and differentiation of lymphocytes in intestinal mucous membranous immune tissues, which will lead to the depression of mucous membranous immune barrier.
BACKGROUND: Transforming growth factor β1(TGFβ1) is one of many cytokines that tumor can autocrine or paracrine to promote cell growth. Accordiug to the recent study, proliferation of osteosarcoma cells was decreased after osteosarcoma cells were transfected by antisense TGFβ1 gene.OBJECTIVE: To investigate the reversing effects of antisense TGFβ1 gene on the malignant phenotypes of osteosarcoma cells and explore the significance of the prevention and treatment of the occurrence and development of osteosarcnma.DESIGN: A completely randomized design and control study were performed.SETTING and MATERIALS: The experiment was carried out in the Orthopaedic Laboratory of Affiliated Union Hospital, Tongji Medical College of Huashong University of Science and Technology. TaqDNA polymerase, TRIzol^TM Reagent , Superscript Preamplificatinn System, fetal bovine serum,Dubecco‘s Modified Eagle medium(DMEM), Lipofectamine, serum-free culture medium, G418, PCR primer, express plasmid of antisense TGFβ1 gene [pcDNA3-TGFβ1( - )], MG-63, Balb/c nude mice, flow cytometry, CO2 Incubator, and phase contrast microscope were used in the experiment.METHODS: After MG-63 cells were transfected by antisense TGFβ1 gene,then the cell cvcle, apoptosis and the expression of matrix metalloproteinase-2(MMP-2) were assayed, and the amount of soft agar colony and the ability of nude mouse inoculation were observed.MAIN OUTCOME MEASURES: Cell cycle assay and apoptosis assay;PT-PCR assay and assay of gene expression of MMP-2 soft agar colony formation, and nude mouse inoculation.RESULTS: Compared with MG-63 and MG-pcDNA3, the amount of cell in the G0/G1 phase of MG-TGFβ1( - )was increased from 56.2% and 60. 1% to 71.6% but that of cell in the S phase was decreased from 19. 1% and 17.8% to 12.9%, and the expression of MMP-2mRNA was decreased obviously. The colony formation rate of MC-TGFβ1 ( - ) was decreased from 48.6±8.1 and 45.2±4.7 to 28.2±5.6. Furthermore, tumor volume (83.4±27.4mm^3) was evidently less than that of MG-63(191.3±21.5mm^3) and MG-pcDNA3 groups(204.2 ± 30. 7mm^3). CONCLUSION: The antisense TGFβ1 gene can reverse the malignant phenntype of osteosarcoma cells to some extent and inhibit tumor invasion and metastasis.
BACKGROUND: Matrix melloproteinases play a pivotal role in tumor invasion and metastasis, but little is known about the correlation between their expression and the prognosis of breast cancer.OBJECTIVE: To investigate the expression of matrix melloproteinases-2 and matrix melloproteinases-9 (MMP-2/MMP-9) and the distributive form of CollV (type IV collagen)in the tissues of breast carcinoma, and find out their relationship with tumor invasion and metastasis so as to disclose the mechanism of the tumor invasion and metastasis of breast carcinoma.SETTING and PARTICIPANTS: Eighty-two filed wax masses of samples from the removed breast carcinoma and 30 filed wax masses of samples from the control group were collected at random from the First Affiliated Hospital of Fujiatt Medical University.MAIN OUTCOME MEASURE: The expression of MMP-2, MMP-9 and the distributive form of CollV in the tissues of breast carcinoma and in the tissues without breast carcinoma, and the correlation of the results with clinieopathological tumor parameters.METHODS: Tissues were obtained from 82 patients with breast carcinoma and 30 without breast carcinoma. The expression of MMP-2, MMP-9 and the distributive form of ColIV were examined, and their correlation with clinicopathological tumor parameters was explored.RESULTS: ① The positive incidences of MMP-2 and MMP-9 expression in 82 cases of breast carcinoma were 52 % (43/82) and 54% (44/82) respectively, The distributive form of encapsulated CollV was 15% (12/82),and the distributive form of decomposed CollVwas 85% (70/82), which were different apparently from those of the control group, ② The expressions of MMP-2/MMP-9 were related to the distribution form of ColIV( P < 0.05).③The expression of MMP-9 was related with the metastasis of the axillary lymph node (X^2 =8, 1899, P < 0, 05), and the expressions of MMP-2 was not related with the metastasis of the axillary lymph node(X^2 = 3. 3590, P>0.05),CONCLUSION: MMP-2, MMP-9 play a pivotal role in tumor invasion and metastasis. The result is suggested that detection of MMP-2/MMP-9 helps to judge the malignant severity of breast carcinoma and its biological behavior.
目的：对准分子激光屈光性角膜切削术(photorefractive keratectomy，PRK)和准分子激光原位角膜磨镶术(laser in situ keratomeleusis，LASIK)联合可蚀盘矫治远视进行观察比较。方法：使用可蚀盘联合三棱镜抛光技术，对25例患者40只远视眼(PRK16眼，LASIK24眼)进行矫治。术前屈光度(+1.62～+6．25)D。对两种术式屈光度、视力等进行比较。结果：随访时间12个月。最佳矫正远视力丢失两行以上PRK组2眼(12．5％)，LASIK组3眼(12．5％)；最佳矫正近视力丢失两行以上PRK组1眼(6．3％)，LASIK组2眼(8．3％)。平均裸眼远视力PRK组为0．55，LASIK组为0．68；而平均裸眼近视力PRK组为0．66，LASIK组为0．85。残余屈光度在-1．00D～+1．00D之间PRK组12眼(75．0％)，LASIK组20眼(83．3％)。平均回退量术后1～3个月，PRK组为0．95D，LASIK组为0．76D；术后3～6个月，两组分别为0.35和0．08D；术后6．12个月，两组分别为-0．22和0．00D。在各阶段中两种术式比较差异均有显著意义。结论：使用可蚀盘联合三棱镜矫治远视是安全的，PRK与LASIK两者方法均可获得满意的远期疗效。但LASIK较PRK更容易稳定且有效性和预测性更好。
由于脊柱本身解剖结构的复杂性，目前脊柱手术的准确定位在很大程度上依赖于术者的经验、解剖知识及三维空间想像能力。人们认识到随着计算机技术的成熟，在提高手术安全性方面脊柱三维成像技术有其独特的应用价值。利用颅脑外科中成熟的无框架立体定向影像系统，结合脊柱本身解剖结构的特殊性，尝试通过计算机辅助导航系统(Computer-assisted Surgery CAS)来帮助脊柱手术中的准确定位，并通过临床应用检验其准确性，将显示其广阔的应用前景。