Effectiveness of dry needling on reducing pain intensity in patients with myofascial pain syndrome： a Meta-analysis ObjectiveTo 在 myofascial 疼痛地在个人减轻疼痛和运动( ROM )的增加的范围上关于干燥刺破的有效性( DN )总结文学从2000年1月出版到2013年1月的症候群( MPS ) .MethodsPapers 在数据库 MEDLINE 通过电子搜索被识别， Dialnet ， Cochrane 图书馆加，物理疗法证据数据库( PEDro )和科学研究( CSIC )的西班牙的优异委员会。包括的研究被使随机化控制了与潜在地相关的 19 临床的审判的 MPS.ResultsOut 在个人在疼痛和 ROM 上关于 DN 的有效性用英语或西班牙语写的审判， 10 的一个总数在元分析被包括。当以前并且立即在干预以后测量了时，关于疼痛紧张减小， DN 与安慰剂治疗相比完成了改进[d =−
0.49；95% CI (−
3.21， 0.42 )] 并且与控制组一起[d =−
9.13；95% CI (−
3.56 )] 。然而，另外的处理与 DN 相比在一样的变量上完成了更好的结果，就为预告的处理并且立即的大小在以后而言[d = 2.54；95% CI (−
0.40， 5.48 )] ，以及预告的处理并且在 3-4 星期以后[d = 4.23；95% CI (0.78， 7.68 )] 。DN 看了显著地增加的 ROM 什么时候在干预前并且立即测量了在以后，与安慰剂比较[d = 2.00；95% CI (1.60， 2.41 )] 。然而，另外的处理关于 ROM 完成了重要更好的结果它什么时候在干预前并且立即被测量在以后，作为与 DN 相比[d =−1.42；95% CI (−
0.99 )].ConclusionDN 在比作安慰剂组的减少的疼痛上是不太有效的。另外的治疗是比在 3-4 星期以后减少疼痛上的 DN 更有效的。在增加 ROM 以后，然而， DN 是更有效的把处理比作安慰剂组，而是不到其它的。
Effect of compound Kushen injection on T-cell subgroups and natural killer cells in patients with locally advanced non-small-cell lung cancer treated with concomitant radiochemotherapy ObjectiveTo 与与随机划分的伴随物 radiochemotherapy.MethodsWe 治疗的局部地先进的 non-small-cell 肺癌症( NSCLC )在病人在T房间亚群和 NK 房间上观察复合 Kushen 注射的效果在我们在2011年5月和2013年5月之间的医院被对待进一个治疗组和控制的有局部地先进的 NSCLC 的 60 个病人组织由绘画。治疗组( n = 30 )加复合 Keshen 注射的收到的伴随物 radiochemotherapy ，和控制组织( n = 30 )接受仅仅 radiochemotherapy.ResultsAfter 治疗，CD3 +的层次，CD4 +， CD4+/CD8 +和房间显著地增加了的CD16+/CD56+，和CD8+房间显著地减少了，在与两个相比，他们的预告的处理铺平的治疗组并且与在控制组的层次。在控制组， CD3 + 的处理以后的层次， CD4 + ， CD4 +/CD8 + 和 CD16+/CD56+ ，房间显著地没从预告的处理层次被改变。二个组显著地没处于他们毒性反应的率不同(P > 0.05 ) 在收到伴随物 radiochemotherapy 的有局部地先进的非小的房间肺癌症的病人的 .ConclusionCompound Kushen 注射罐头增加 immunologic 功能。
Acupuncture-movement therapy for acute lumbar sprain： a randomized controlled clinical trial OBJECTIVE: Several studies have reported that acupuncture is effective for treatment of acute lumbar sprain, but they neglected to consider that acupuncture cannot remarkably improve lumbar activity. We performed a randomized controlled trial to evaluate the effect of acupuncture-movement therapy versus conventional acupuncture in the treatment of acute lumbar sprain.METHODS: Sixty patients were randomized into four groups: the acupuncture-movement(AM)group, sham acupuncture-movement(SAM) group,conventional acupuncture(CA) group, and physical therapy(PT) group. Patients in the AM group were treated with acupuncture at Yintang(EX-HN 3) and exercise of the lumbar region during acupuncture.Patients in the SAM group were treated with sham acupuncture at Yintang(EX-HN 3) and exercise of the lumbar region during sham acupuncture. Conventional acupuncture was performed in the CA group, and physical therapy was applied in the PT group. Each treatment lasted for 20 min. Patients were assessed before and after treatment using a visual analogue scale(VAS) and the Roland Morris Questionnaire(RMQ).RESULTS: The VAS and RMQ scores in the AM group were significantly lower after than before treatment(P < 0.01). The AM group reported lower RMQ scores in after-treatment and 24 h after treatment and lower VAS score in 24 h after treatment in comparison with those of the CA, SAM, and PT groups(P < 0.05).CONCULSION: Acupuncture-movement therapy is effective for treating acute lumbar sprain with a persistent pain-relief and remarkable improvement of lumbar activity. Movement, that is, lumbar exercise during acupuncture, enhances the effect of acupuncture.
Effect of bloodletting therapy at local myofascial trigger points and acupuncture at Jiaji （EX-B 2） points on upper back myofascial pain syndrome： a randomized controlled trial OBJECTIVE: To observe the clinical efficacy of bloodletting therapy and acupuncture at Jiaji points for treating upper back myofascial pain syndrome(MPS), and compare this with lidocaine block therapy.METHODS: A total of 66 upper back MPS patients were randomly assigned to either the treatment group or the control group in a 1∶1 ratio. The treatment group(n = 33) were treated with bloodletting therapy at local myofascial trigger points and acupuncture at Jiaji(EX-B 2) points; one treatment course consisted of five, single 20-min-treatments with a 2-day break between each treatment. The control group(n = 33) were treated with a lidocaine block at trigger points; one treatment course consisted of five sessions of lidocaine block therapy with a 2-day break between each session. The simplified Mc Gill Scale(SF-MPQ) and tenderness threshold determination were used to assess pain before and after a course of treatment.RESULTS: After the third and fifth treatment, the SF-MPQ values were significantly decreased(P <0.01) and the tenderness thresholds were significantly increased(P < 0.01) in both groups compared with before treatment. There were no significant differences in pain assessments between the two groups after three and five treatments(P >0.05). There were five cases with minor adverse reactions reported in the control patients, while no adverse reactions were reported in the treatment group.CONCLUSION: Bloodletting therapy at local myofascial trigger points and acupuncture at Jiaji points was effective in treating upper back MPS.Clinically, bloodletting and acupuncture therapy had the same efficacy as the lidocaine block therapy, with fewer adverse reactions.
Effects of Traditional Chinese Medicine herbs for tonifying Qi and kidney, and replenishing spleen on intermittent asthma in children aged 2 to 5 years old OBJECTIVE: To evaluate the efficacy and safety of Traditional Chinese Medicine(TCM) herbs for tonifying Qi and kidney, and replenishing spleen on intermittent asthma in children aged 2 and 5 years.METHODS: A randomized, single-blind, placebo controlled trial was conducted. Children with intermittent asthma were enrolled and their baseline conditions were measured using a questionnaire. A total of 60 participants, aged 2 to 5, were randomized into either the treatment group(n = 40) or the placebo group(n = 20). The treatment group was treated with granules of TCM herbs for tonifying Qi and kidney, and replenishing spleen, and the placebo group was given placebo granules for 3 months.The number of asthma attacks was counted and TCM syndrome scores were measured at 1, 2, 3, 6, 9,and 12 months. The airway resistance and levels of eosinophil cationic protein for the two groups were observed before and after 3 months. The results were statistically analyzed.RESULTS: Compared with the placebo group, the number of asthma attacks significantly decreased in the treatment group(P < 0.05). For the treatment group, the TCM syndrome scores decreased after 1 and 2 months; there was also a significant difference in scores between the two groups(P < 0.05).The difference remained after the medicine was stopped for 9 months(P < 0.05). After the 3-month treatment, compared with the placebo group, the airway resistance decreased in the treatment group(P < 0.05). No adverse events were reported in the treatment group.CONCLUSION: The TCM herbs for tonifying Qi and kidney, and replenishing spleen reduced the number of intermittent asthma attacks, decreased the TCM syndrome scores, and reduced the airway resistance in the children aged 2 to 5.
Efficacy and safety evaluation of intravenous infusion of cervus and cucumis polypeptides for treatment of avascular necrosis of the femoral head： a randomized clinical trial OBJECTIVE: To investigate the efficacy and safety of intravenous cervus and cucumis polypeptides for treating avascular necrosis of the femoral head(ANFH) in regard to pain and hip function in a randomized clinical trial.METHODS: A total of 96 subjects with ANFH who were recruited at the Orthopaedic Hospital Affiliated with Hebei United University and Qian Hai Femoral Head Hospital of Beijing were assigned by lottery to an intervention group(n = 48) or a control group(n = 48). All subjects underwent physical therapy and rehabilitation exercises. In addition,subjects in the intervention group were given intravenous infusions of cervus and cucumis polypeptides. Visual analogue scale(VAS), Harris hip score,and radiography or magnetic resonance imaging were applied to assess all subjects at the beginning of treatment and 3, 6, and 9 months afterward. All the subjects were followed up for 2 years.RESULTS: At the beginning of treatment, there were no statistically significant differences between the two groups in terms of the general condition of patients or the VAS and Harris hip scores(all P > 0.05). At 3, 6, and 9 months after treatment,however, the VAS score decreased and the Harris hip score increased in all patients, with the improvement of intervention group significantly greater than that of the control group(P < 0.05).The total effectiveness rates for the intervention and control groups were 89.58% and 70.83%, respectively, with the difference being statistically significant(P < 0.05). There was no statistically significant difference between the two groups in terms of the safety of the injections(P > 0.05).CONCLUSION: Intravenous infusion of cervus and cucumis polypeptides relieved pain and improved hip function of subjects with ANFH.Thus, the intravenous infusion of cervus and cucumis polypeptides was a safe, effective treatment for ANFH.
Efficacy and safety of Yunxiangjing derived from Chinese herbal medicine administered as an enema in the management of acute radiation-induced proctitis in patients with pelvic malignancy OBJECTIVE: To assess the efficacy and safety of Yunxiangjing(YXJ), derived from Chinese herbal medicine, in the management of acute radiation-induced proctitis(ARIP) in patients with pelvic malignancy.METHODS: Forty-eight patients with grade 2 ARIP were administered YXJ as an enema(1∶30 dilution)for 2 weeks and followed up for 2 years. All were assessed for response and ARIP grade. Quality of life(QOL) was assessed with the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Core 30.RESULTS: Of the 48 patients, six(12.5%) achieved complete remission of ARIP and 28(58.3%) showed a decrease from grade 2 to grade 1 ARIP. No patient experienced a grade ≥3 toxicity. At the end of radiotherapy, patients showed significant improvements in QOL(P < 0.05). Two years after treatment, 46 patients showed no late toxicity, with only two experiencing grade 1 late toxicity.CONCLUSION: YXJ can be used as an enema to manage acute radiation-induced proctitis in cancer patients undergoing radiotherapy. These findings suggest that YXJ enema may be an alternative treatment of ARIP.
Effect of transcutaneous electrical nerve stimulation through acupoints of Pucan （BL 61） and Shenmai （BL 62） on intraocular pressure in patients with glaucoma： a randomized controlled trial ObjectiveTo 调查 transcutaneous 的效果通过在有 glaucoma.MethodsA 的病人的 intraocular 压力(IOP ) 上的 acupoints 的电的神经刺激(十) 使失明单人赛的、使随机化的试验性的设计学习被进行。从与 intraocular 高血压诊断的台北眼科学门诊病人部门的北方的病人被选择并且如果他们满足了研究题目标准，进一步被注册。谁的病人满足了所有标准，并且没满足排除标准的人，成为了参加者。参加者随机被分配进对待的组，在那里 TENS 电极(Qing 明医药设备有限公司，新台北城市，中国) 为 20 min 在 acupoints Pucan (BL 61 ) 和 Shenmai (BL 62 ) 上与直接电流(DC ) 被使用。控制组不收到了十电极治疗。IOP 的大小用 pneumotonometer 被花四次(Clinico 有限公司，新台北城市，中国) ，以前立即在以后， 30 min 在以后，和在十以后的 60 min 电极 treatments.ResultsEighty -- 二个参加者完成了实验。IOP 在与 DC 对待的组更减少了(P < 0.01 ) 比在控制组。lOPs 不受病人的年龄和他们 Pucan (BL 61 ) 的 acupoints 的 medications.ConclusionTENS 的用法影响， Shenmai (BL 62 ) 能显著地与绿内障在病人减少 IOP。
Effect of modified fasting therapy on body weight, fat and muscle mass, and blood chemistry in patients with obesity 学习是调查修改 fasting 治疗使用的效果和安全的这的 ObjectiveThe 目的在身体重量，脂肪和肌肉上使药用的植物和锻练发酵了在肥胖的 subjects.MethodsTwenty 的质量，和血化学--参予的六个病人一14天快，在哪个期间他们摄取了用使发酵的药用的植物和糖类做的补充(吸入：400-600 kcal/d ) 。时间表包括了 7 prefasting 消除天和 14 天食物的逐步的再介绍。病人也参加了合并了 Qigong 的一个锻练节目，训练，并且走的重量锻练。治疗的功效被估计身体脂肪质量和肌肉观察质量，和丙氨酸 aminotransferase， aspartate aminotransferase (著名计算机生产厂商) ，胆固醇，和在各个的 triglycerides (中高音) 学习经期。特定的症状或副作用是 reported.ResultsBody 重量和身体脂肪质量两个都显著地减少了由(5.16 ±
0.95 ) 并且(3.89 ±
0.79 ) kg (两 P < 0.05 ) ，当肌肉质量由一般水准减少了时(0.26 ±
0.22 ) kg，没有统计意义。中高音层次显著地被减少(P < 0.05 ) ，当没有统计意义，著名计算机生产厂商层次减少了时(P = 0.052 ) 。全部的胆固醇和 triglycerides 的层次显著地也被减少(两 P < 0.05 ) 。除了温和头昏有很少不利事件，它没影响结果建议的日常 living.ConclusionThese 用使发酵的药用的植物和锻练的那修改 fasting 治疗能在肥胖的病人上有效、安全。
Pulse changes in patients with cervical spondylosis before and after acupuncture treatment OBJECTIVE: To study changes in pulse diagram parameters(PDP) in patients with cervical spondylosis(CS) before and after acupuncture treatment, explore the characteristics of PDP and the relationship between PDP changes and therapeutic effectiveness, and provide evidence for outcome prediction and objective evaluation of CS treatment before and after acupuncture treatment.METHODS: Patients with CS were treated with acupuncture and measured with a pulse acquisition device based on image(PADBI) before the first and after the tenth acupuncture sessions. Changes in PDP from before until after the acupuncture sessions and patient impressions were analyzed to judge the effect of acupuncture treatment for.RESULTS: The PDP values in effective patients were closer to normal values. This indicated that Qi stagnation and blood stasis of the patients was improved. The PDP changes from before to after the first acupuncture treatment were more obvious than those from before to after the tenth acupuncture treatment. This result indicates that the speed of symptom improvement decreased significantly after several acupuncture courses. Analysis of correlation between efficacy and PDP showed that the changes in PDP in five patients was abnormal,which mainly manifested as values of h1, u, p, Pp,and t1, and no significant changes or differences were increased with standard values. This indicated that the symptoms of CS were not improved in these patients.CONCLUSION: PADBI can provide evidence for outcome prediction of acupuncture treatment in patients with CS. PADBI can provide evidence for objective evaluation of acupuncture treatment of CS.
Nelumbo Nucifera leaf extract attenuated pancreatic β-cells toxicity induced by interleukin-1β and interferon-y, and increased insulin secrection of pancreatic 13-cells in streptozotocin-induced diabetic rats OBJECTIVE: To evaluate the effect of Nelumbo Nucifera leaf water extract(NNLE) on insulinoma(RIN)cells induced by interleukin-1β(IL-1β) and interferon-g(IFN-γ), and injured pancreatic β-cells induced by Streptozotocin(STZ) in rats.METHODS: The anti-oxidative effects of NNLE were assessedusing 1,1-diphenyl-2-picrylhydrazyl(DPPH)and nitric oxide(NO) scavenging assays. The inhibitory effect of NNLE on α-glucosidase and DPP(dipeptidyl peptidase)-IV was measured in vitro. Pancreatic β-cell protective and insulin secretory effects were assessed, using IL-1β and IFN-γ-induced rat RIN cells. STZ-induced diabetic rats were treated with 50, 100, and 400 mg/kg NNLE for 4 weeks. The effects of NNLE on blood glucose(BG), body weight(BW), and lipid profiles were measured.RESULTS: NNLE inhibited DPPH, NO, α-glucosidase,and DPP-Ⅳ which were directly linked to the function of β-cells. Furthermore, NNLE protected RIN cells from toxicity induced by IL-1β and IFN-γ, decreased NO production, and increased insulin secretion. NNLE caused a significant reduction in blood glucose, triglyceride(TG), total cholesterol(TC),blood urea nitrogen(BUN), and creatinine in STZ-induced diabetic rats. Furthermore, it significantly decreased BW loss in STZ-induced diabetic rats.CONCLUSION: Our results suggest that NNLE reduced the toxicity in insulinoma cells and increased insulin secretion in pancreatic β-cells in STZ-induced diabetic rats.
Puerarin reduces apoptosis in rat hippocampal neurons cultured in high glucose medium by modulating the p38 mitogen activated protein kinase and c-Jun N-terminal kinase signaling pathways OBJECTIVE: To investigate the neuroprotective effect of puerarin on rat hippocampal neurons cultured in high glucose medium, and to examine the role of the p38 mitogen activated protein kinase(p38 MAPK) and c-Jun N-terminal kinase(JNK) signaling pathways in this effect.METHODS: Primary cultures of hippocampal neurons were prepared from newborn Sprague Dawley rats. Neuron-specific enolase immunocytochemistry was used to identify neurons. The neurons were cultured with normal medium(control group) or with high-glucose medium(high-glucose group),and puerarin(puerarin group), a p38 MAPK inhibitor(SB239063; p38 MAPK inhibitor group) or a JNK inhibitor(SP600125; JNK inhibitor group) were added. After 72 h of treatment, terminal deoxynucleotidyl transferase-mediated d UTP nick end labeling assay was performed to detect apoptosis, and western blotting was used to assess protein levels of p-p38, p38, p-JNK and JNK.RESULTS: In the high-glucose group, the neuronal apoptosis rate and the p-p38/p38 and p-JNK/JNK ratios were higher than in the control group. The p38 MAPK and JNK inhibitors prevented this increase in the apoptosis rate. The apoptosis rates in the puerarin group, the p38 MAPK inhibitor group and the JNK inhibitor group were significantly decreased compared with the high-glucose group.Moreover, protein levels of p-p38 and p-JNK were significantly reduced, and the p-p38/p38 and p-JNK/JNK ratios were decreased in the puerarin group compared with the high-glucose group. In addition, compared with the high-glucose group,p-p38 levels and the p-p38/p38 ratio were reduced in the p38 MAPK inhibitor group, and p-JNK levels and the p-JNK/JNK ratio were decreased in the JNK inhibitor group.CONCLUSION: Puerarin attenuates neuronal apoptosis induced by high glucose by reducing the phosphorylation of p38 and JNK.
Electroacupuncture alters pain-related behaviors and expression of spinal prostaglandin E2 in a rat model of neuropathic pain OBJECTIVE: To investigate the role of spinal prostaglandin E2（PGE2） in electroacupuncture（EA） analgesia and assess the theoretical basis for selection of acupoints in the treatment of neuropathic pain.METHODS: A rat model of neuropathic pain was established. Rats were randomly divided into normal,model, sham, EA 1, EA 2, and EA 3 groups. In EA 1group, the rats were needled at bilateral L5 Jiaji（EX-B2）, Dachangshu（BL 25）, Weizhong（BL 40） and Kunlun（BL 60）. In EA 2 group, the rats were needled at bilateral Weizhong（BL 40） and Kunlun（BL60）. In EA 3 group, the rats were needled at bilateral L5 Jiaji（EX-B2） and Dachangshu（BL 25）. EA stimulation was administered once daily over 7 days. Motor function and thermal withdrawal latencies were evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. After 7 days of intervention,enzyme-linked immunosorbnent assay（ELISA） was used to quantify the expression of the spinal PGE2.RESULTS: Rats in the model group exhibited evident hyperalgesia in responses to thermal withdrawal latencies compared with those in the control group（P < 0.01）, and EA reversed thermal withdrawal latencies（P < 0.01）. The expression level of the spinal PGE2 was significantly higher in the model group than that in the control group and was reversed by EA（P < 0.01; P < 0.05）.CONCLUSION: The effect of EA on neuropathic pain might alleviate the hyperalgesia state by an inhibition of local prostaglandin E2 secretion.
Effect of Xinfeng capsule on nuclear factor kappa B/tumor necrosis factor alpha and transforming growth factor beta 1/Smads pathways in rats with cardiac injuries induced by adjuvant arthritis OBJECTIVE: To investigate effects of Xinfeng capsule(XFC) on cardiac function in rats with adjuvant arthritis(AA) and explore the mechanism of these effects.METHODS: Forty-eight rats were randomly divided into normal control(NC), model control(MC), methotrexate(MTX) and XFC groups of equal size. In all groups except for the NC group, 0.1 m L Freund’s complete adjuvant(FCA) was intracutaneously injected in the right rear vola pedis to induce inflammation. Drugs were applied beginning 19 days after induction of inflammation. Normal saline was administered to the NC and MC groups and 1 mg/100 g MTX(weekly) and 0.12 g/100 g XFC(daily) to the MTX and XFC groups, respectively. Rats were sacrificed after 30 day of treatment. Toe swelling degree(TSD), arthritis index(AI), cardiac function and expression of nuclear factor kappa B(NF-κB) / tumor necrosis factor alpha(TNF-α) and transforming growth factor beta 1(TGF-β1)/Smads pathway proteins were measured.RESULTS: In the MC group, TSD and AI were greatly increased, while parameters of cardiac function were decreased and morphological analysis showed myocardial cell damage. Expression of TNF-α, NF-κB, Smad2, P-Smad2, Smad4 and TGF-β1 proteins were elevated in cardiac tissue, while Smad7 expression was decreased.TSD and AI values closely correlated to parameters of cardiac function and to levels of proteins in the NF-κB/TNF-α and TGF-β1/Smads pathways. Certain correlations were identified among TGF-β1 and NF-κB, Smad2, P-Smad2 and Smad4.With XFC intervention, both TSD and AI were decreased and parameters of cardiac function and ultrastructure of myocardial cells improved.Expressions of NF-κB, Smad2, and Smad4 proteins were greatly decreased and Smad7 expression was elevated, as compared with levels in the MC and MTX groups.CONCLUSION: XFC regulates expression of proteins in the NF-κB/TNF-α and TGF-β1/Smads pathways, decreases immune complex deposition in cardiac tissue and improves cardiac function in AA rats via upregulatio...Effect of Gubenfangxiao decoction on respiratory syncytial virus-in duced asthma and expression of asthma susceptibility gene oroso- mucoid 1-like protein 3 in mice OBJECTIVE: To investigate the effect of Gubenfangxiao decoction(GBFXD) on respiratory-syncytial-virus(RSV)-induced asthma and the expression of asthma susceptibility gene, orosomucoid 1-like protein 3(ORMDL3) in mice.METHODS: Seventy-two female BALB/c mice were randomly assigned to normal, model, GBFXD high dose, GBFXD moderate dose, GBFXD low dose and montelukast groups. An asthma model was induced via intraperitoneal injection and aerosol inhalation of ovalbumin(OVA) and repeated intranasal instillation of RSV in all mice, except those in the normal group. All treatments were administered at the first onset of asthma(within 8 weeks of model establishment) and the mice were euthanized after 28 days of treatment. The levels of transforming growth factor-β(TGF-β) and interleukin-6(IL-6) in bronchoalveolar lavage fluid(BALF) of the mice were measured and the expression of asthma susceptibility gene ORMDL3 in lung tissue was determined using real-time polymerase chain reaction(RT-PCR) and western blotting.RESULTS: Expression of ORMDL3 and levels of TGF-β and IL-6 were significantly higher in the model group(P < 0.05, P < 0.01) compared with the normal mice. Levels of ORMDL3, TGF-β and IL-6 were significantly lower in all three GBFXD treated groups(P < 0.05) compared with the model group.However, the levels in the GBFXD treatment groups did not differ significantly from the montelukast group.CONCLUSION: GBFXD had a therapeutic effect in this experimental model. The functional mechanism of GBFXD may involve multiple factors, including alleviation of airway inflammation, down-regulation of asthma susceptibility gene ORMDL3 and inhibition of airway remodeling.
Bloodletting at Jing-well points decreases interstitial fluid flow in the thalamus of rats
Synergetic effects of aqueous extracts of Fuzi （Radix Aconiti Lateralis Preparata） and Tubeimu （Rhizoma Bolbostemmatis） on MDA-MB-231 and SKBR3 cells OBJECTIVE: To test the synergistic effects of the aqueous extract of Tubeimu(Rhizoma Bolbostemmatis) and Fuzi(Radix Aconiti Lateralis Preparata) on MDA-MB-231 and SKBR3 breast cancer cells.METHODS: A combined index was created for the effects of Tubeimu(Rhizoma Bolbostemmatis) and Fuzi(Radix Aconiti Lateralis Preparata) extracts. Cell proliferation was performed by trypan blue exclusion and 3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assays. Flow cytometry was used to assess cell cycle distribution and apoptosis. Cell migration was determined by wound-healing and transwell assays. Confocal microscopy was used to detect E-cadherin and actin filaments.RESULTS: The aqueous extract from Tubeimu(Rhizoma Bolbostemmatis) and Fuzi(Radix Aconiti Lateralis Preparata) exerted synergetic effects on the growth of MDA-MB-231 cells and G1 phase arrest.When exposed to extracts at concentrations of 62.5∶62.5 and 62.5∶31.3 μg/m L, the combination index was 0.83 and 0.74, respectively. Interestingly, 62.5∶31.3 μg/m L of combined drugs enhanced the inhibitory effect of Tubeimu(Rhizoma Bolbostemmatis)on the migration of SKBR3 cells and reduced the stimulative effect of Fuzi(Radix Aconiti Lateralis Preparata)(P < 0.01), in which cells showed an increased expression of E-cadherin and reorganization of actin filaments(P < 0.001). 62.5∶62.5 μg/m L extract also synergistically induced apoptosis of MDA-MB-231 cells(P < 0.05 and P < 0.001). Acting as the main active ingredients in the extract, tubeimoside I and acetylbenzoylaconine at 10∶10 μg/m L and 5∶2.5 μg/m L also produced inhibitory effects on the proliferation and migration of cells(P <0.01).CONCLUSION: Tubeimu(Rhizoma Bolbostemmatis)and Fuzi(Radix Aconiti Lateralis Preparata) extracts had synergic effects on MDA-MB-231 and SKBR3 cells.
Ocular myasthenia gravis accompanied by anosmia 我们报导嗅觉缺失伴随的眼睛的重症肌无力(MG ) 的一个案例。一个 76 岁的人有 2 年的持续时间的自发的嗅觉缺失。在会诊前的四个月，他开始让了在项与肌肉痛苦，扩张，和疼痛一起在恰好上面的眼皮低垂。他的舌头与薄、白的涂层是黑暗红的；他的脉搏金属线制、滑溜溜。根据繁体中文药，眼皮低垂和嗅觉缺失是肝限制和怒气缺乏的主要符号。在西方的药，诊断是眼睛的 MG 和自发的嗅觉缺失。与文学一起，我们的病人建议那嗅觉缺失可以是在 MG 前的早症状。嗅觉缺失伴随的 MG 能根据抗体生产和症状是 MG 的一种特殊子类型。
主 编：hu ximing