Different endometrial preparation protocols yield similar pregnancy outcomes for frozen-thawed embryo transfer in patients with advanced endometriosis Objective To explore the different endometrial preparation for frozen-thawed embryo transfer(FET) in women with advanced endometriosis(EMS).Methods The pregnancy outcomes of patients with advanced EMS(542 cycles), who were prepared for FET, were retrospectively assessed. Included patients underwent a total of 233 FET cycles(180 patients) using natural cycle(NC), a total of 142 FET cycles(115 patients) using letrozole(LE) ovulation induction, and a total of 167 FET cycles(137 patients) using hormonal replacement treatment(HRT) for endometrial preparation.Results There were no significant diffenences in the clinical pregnancy rate(LE:49.30%, NC: 50.21%, and HRT: 43.11%, P=0.343), the implantation rate(LE: 29.26%,NC: 36.03%, and HRT: 29.55%, P=0.084), and the live birth rate(LE: 38.02%, NC:39.11%, and HRT: 35.33%, P=0.648) among the three groups. No statistically significant differences were observed in the ongoing pregnancy rate, the miscarriage rate,and the pregnancy complication rate. The single birth weight in patients using NCFET was lower than that in patients using HRT-FET(P=0.044) and a higher twin birth weight in patients using LE-FET were observed compared with other groups(P=0.022).The rate of birth weight <2 500 g was also higher in the NC-FET group than in other groups. No congenital birth defects were found in the three groups.Conclusion Different endometrial preparation protocols without ultra-long Gn RH-adown-regulation for FET yield similar pregnancy outcomes in patients with EMS. A tailored endometrial preparation protocol should be recommended according to different patients?situation.
A preliminary observation on efficacy of growth hormone on poor ovarian responders in IVF/ICSI cycles Objective To determine efficacy of gonadotropin(Gn) co-treated with growth hormone(GH) on poor ovarian response(POR) patients undergoing in vitro fertilization/intracytoplasmic sperm injection(IVF/ICSI).Methods A total of 58 IVF/ICSI-embryo transfer(ET) cycles in POR patients were retrospectively identified. The POR criteria were defined by Bologna consensus. The cycles were divided into two groups: Gn co-treated with GH group(GH+ group, 25 cycles)and Gn only group(GH- group, 33 cycles). Results of IVF/ICSI for these two groups were compared.Results The number of oocyte retrieved and the normal fertilization rate in the two groups were no difference(P>0.05). High-quality embryo rate and implantation rate in the GH+ group were 39.6% and 38.5%, respectively, which were slightly higher than those in the GH-group(35.9% and 25.0%), but no difference was found on these two parameters(P>0.05).Conclusion GH, to some extent, can improve the quality of embryo and the implantation rate. Whether GH co-therapy has a definite role in improving the outcome of POR patients still needs further evaluation.更多还原
Expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion and its correlation with chromosome segregation Objective To investigate the expression of centromere protein-C(CENP-C) in villus tissue of the first-trimester spontaneous abortion(SA) and the correlation study of CENP-C expression with chromosome segregation.Methods Fluorescence in situ hybridization(FISH) and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group(n=30) and control group(n=30) according to the results with FISH. The q RT-PCR and Western blotting analysis were used to assess the expression level of CENP-C.Results Forty-eight(51.06%) cases had observed the numerical chromosome abnormality, including 30 positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C m RNA and protein in case group were statistically higher than that in control group(P<0.05).Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid.
Papanicolaou smear interpretation with progestin-only hormonal birth control methods Objective To define the relationship between the use of progestogen-only contraception and the occurrence of abnormal cervical cytology and cervical intraepithelial lesion.Methods The present study was designed as a case-control study including 200progestogen-only contraceptive(POC) users recruited as cases(group A) and 50 women who were non-hormonal contraceptive users as a control group(group B). Positive Papanicolau(Pap) smear was reported in cervical cell abnormalities from atypical squamous cell of undetermined significance(ASCUS) to invasive cancer. Pap smear was evaluated according to the Bethesda system classification 2001. Cases with positive Pap smears were subjected to visual inspection of the cervix with 5.0% acetic acid(VIA test) and punch biopsy.Results Statistical analysis of Pap smear results in this study showed epithelial cell abnormalities in 44 cases(22.0%) in group A and 9 cases(18.0%) in group B showing no statistically significant difference(P>0.05). ASCUS abnormality was reported in31 cases(15.5%) of group A and 6 cases of group B(12.0%). Low-grade squamous intraepithelial lesion(LSIL) was found in 11 cases(5.5%) and 3 cases(6.0%) of group A and group B, respectively. High-grade squamous intraepithelial lesion(HSIL) was found in 2 cases(1.0%) of group A and none of group B. The incidence of abnormal Pap smears in group A did not greatly vary between different methods of POCs. Among Depo medroxyprogesterone acetate(DMPA) users, ASCUS was found in 17 cases(16.8%), LSIL was found in 11 cases(10.8%) and HSIL was found in 2 cases(1.9%).While in progesterone-only pills(POPs) users and Implanon?users, ASCUS was found in 10 cases(12.8%) and 4 cases(20.0%) respectively with no higher epithelial abnormalities found. Levonorgestrel intrauterine device(LNG-IUD) was used by 1 casein group A and was not associated with Pap smear abnormality. Cases with ASCUS abnormality were followed up by repeated Pap smear after 3 months. Twenty cases(64.5%) and 3 cas...Status of reproductive health knowledge for junior high school students in Guangzhou city Objective To understand the status of reproductive health knowledge for junior high school students in Guangzhou city and provide the base data for reproductive health education.Methods A total of 1 210 students including 657 boys and 553 girls aged 12-15 years were surveyed with anonymous self-administrated questionnaire. The students were from a middle school in suburb of Guangzhou city. The questionnaire contained basic concepts of reproduction, love, sex, contraceptives, abortion and sexually transmitted diseases(STDs). Analysis consisted of descriptive statistics and Chi square tests for differences between boys and girls.Results The students mostly had experienced menarche(94%) or first spermatorrhea(83.6%). About 58.4% of students just knew little about process of puberty, fertilization,and contraception. Far more boys than girls considered that the effect of abortion on physical injury was harmless(30.4% and 9.6%, P<0.001). More than 60% of respondents knew the route of transmission of STDs well, but were poor understanding of adverse impact of STDs. The majority of the students did not consider sexual relationship acceptable at their age, a viewpoint that was more strongly held by girls(61.6%)than boys(45.1%)(P<0.001). Just 35.5% of boys would refuse when the lovers required to have a sex activity, far less than girls(76.1%)(P<0.001). The rate of sex intercourses among students was 5%, and a slightly higher proportion(46%) had neverused contraception among those who had sexual activity. The demands of students on reproductive health were mainly on how to deal with the relationship with lovers(56.0%),knowledge on sexual health and menses(41.7%), sex moral knowledge(48.3%),contraception(45.0%), and preventive measures of STDs/acquired immune deficiency syndrome(AIDS)(47.0%).Conclusion The reproductive health knowledge for the students was at very low level.There were some differences on the knowledge mastered by boys and girls while the sexual opinion of b...Endoplasmic reticulum stress-induced apoptosis in the development of reproduction Proteins synthesized in the endoplasmic reticulum(ER) are properly folded with the assistance of ER chaperones. Accumulation of misfolded protein in the ER triggers an adaptive ER stress(ERS) response termed the unfolded protein response. Recent interest has focused on the possibility that the accumulation of misfolded proteins can also contribute to reproductive response, including preimplantation embryos, testicular germ cell, placenta, and unexplained intrauterine growth restriction(IUGR). The major ERS pathway constituents are present at all stages of preimplantation development and that the activation of ERS pathways can be induced at the 8-cell, morula and blastocyst stage. This review mainly introduced the research progress of ERS induced apoptosis of reproductive cells, providing a new direction for the research of reproductive disease therapy.
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