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  • Immobilized enzyme reactors in HPLC and its application in inhibitor screening:A review
  • This paper sets out to summarize the literatures based on immobilized enzyme bio-chromatography and its application in inhibitors screening in the last decade.In order to screen enzyme inhibitors from a mass of compounds in preliminary screening,multi-pore materials with good biocompatibility are used for the supports of immobilizing enzymes,and then the immobilized enzyme reactor applied as the immobilized enzyme stationary phase in HPLC.Therefore,a technology platform of high throughput screening is gradu...
  • Determination of drug,excipients and coating distribution in pharmaceutical tablets using NIR-CI
  • The growing interest of the pharmaceutical industry in Near Infrared-Chemical Imaging (NIR-CI) is a result of its high usefulness for quality control analyses of drugs throughout their production process (particularly of its non-destructive nature and expeditious data acquisition).In this work,the concentration and distribution of the major and minor components of pharmaceutical tablets are determined and the spatial distribution from the internal and external sides has been obtained.In addition,the same NI...
  • Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation
  • Electrochemical reduction behavior of Adefovir was studied using Hanging Mercury Drop Electrode (HMDE) in Britton-Robinson (BR) buffer solution.Voltammetric study showed one well-defined reduction peak in the potential range -1.2 to -1.4 V (vs. Ag/AgCl) due to reduction of C=N bond of the imidazole ring.Solid-phase extraction and protein-precipitation techniques were employed for extraction of Adefovir from human plasma.The proposed method allows quantification of Adefovir in human plasma over the concentra...
  • Validated stability indicating methods for determination of nitazoxanide in presence of its degradation products
  • Three sensitive,selective and reproducible stability-indicating methods are presented for determination of nitazoxanide (NTZ),a new anti-protozoal drug,in presence of its degradation products.Method A utilizes the first derivative of ratio spectra spectrophotometry by measurement of the amplitude at 364.4 nm using one of the degradation products as a divisor.Method B is a chemometric-assisted spectrophotometry,where principal component regression (PCR) and partial least squares (PLS) were applied.These two ...
  • The expert system of genotype discrimination for D5S818 locus based on near-infrared spectroscopy-principal discriminant variate
  • This paper studied the expert system of genotype discrimination for the STR locus D5S818 based on near-infrared spectroscopy-principal discriminant variate (PDV).Six genotypes,i.e.genotypes 10-10,10-11,11-11,11-12,11-13 and 13-13,were selected as research subjects.Based on the optimum polymerase chain reaction (PCR) conditions,about 54 measuring samples for each genotype were obtained;these samples were tested by near-infrared spectroscopy directly.With differences between homozygote genotypes and heterozyg...
  • Voltammetric behavior of sedative drug midazolam at glassy carbon electrode in solubilized systems
  • Redox behavior of midazolam was studied at a glassy carbon electrode in various buffer systems,supporting electrolytes and pH using differential pulse,square-wave and cyclic voltam-metry.Based on its reduction behavior,a direct differential pulse voltammetric method has been developed and validated for the determination of midazolam in parenteral dosage.Three well-defined peaks were observed in 0.1% SLS,Britton-Robinson (BR) buffer of pH 2.5.The effect of surfactants like sodium lauryl sulfate (SLS),cetyl t...
  • Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein
  • A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum a-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA).A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents,less total assay time,and better linearity,recovery,precision,sensitivity and validity.AFP was detected in forty human serum samples by the proposed M...
  • Development and validation of RP-HPLC and RP-UPLC methods for quantification of parathyroid hormones (1-34) in medicinal product formulated with meta-cresol
  • Rapid and sensitive reversed phase high performance liquid chromatography (RP-HPLC) and ultra performance liquid chromatography (RP-UPLC) method with UV detection has been developed and validated for quantification of parathyroid hormone (PTH) in presence of meta-cresol as a stabilizer in a pharmaceutical formulation.Chromatography was performed with mobile phase containing 0.1% Trifluoroacetic acid (TFA) in MilliQ water and 0.1% TFA in acetonitrile with gradient program and flow rate at 0.3 mL/min for HPLC...
  • Separation,identification,and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV,ion trap mass spectrometry,and electrochemical detection
  • The qualitative and quantitative analysis of active constituents in Fructus Psoraleae is presented by high-performance liquid chromatography (HPLC) coupled with different detections.Extracts of Fructus Psoraleae were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of coumarins,benzofuran glycosides,flavonoids,and meroterpene were identified.The determination of four major constituents including bavachin,isobavachalcone,bavachinin,and bakuchiol was accomplished by HPLC with UV...
  • ORIGINAL ARTICLE Development and validation of a normal-phase HPTLC method for the simultaneous analysis of Lamivudine and Zidovudine in fixed-dose combination tablets
  • Simultaneous quantification of Lamivudine and Zidovudine in tablets by HPTLC method was developed and validated.The chromatograms were developed using a mobile phase of toluene:ethyl acetate:methanol (4:4:2,v/v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 276 nm.The R f values were 0.4170.03 and 0.6070.04 for Lamivudine and Zidovudine,respectively.The linearity of the method was found to be within the concentration range of 50 250 ng/spot for ...
  • New fluorimetric assay of horseradish peroxidase using sesamol as substrate and its application to EIA
  • Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as substrate.The principle of the assay is as follow:sesamol (3,4-methylenedioxy phenol) is reacted enzymatically in the presence of hydrogen peroxide to produce dimeric sesamol.The dimer is fluorescent and can be detected sensitively at ...
  • Application of analytical instruments in pharmaceutical analysis
  • GCMS-QP2010 The GCMS-QP2010 Ultra is our most advanced gas chromatograph mass spectrometer. Descended from the GCMS-QP2010 Plus, it features best-in-class scan speed and sensitivity.
  • JPA Prize in 2011
  • The six excellent papers awarded the 2011 JPA Prize were as follows: 1. Hui Liu, Juan Su*, Xu Liang, Xi Zhang, Ya-Jun He, Hai-Qiang Huang, Ji Ye, Wei-Dong Zhang*. Identification and
  • Immobilized enzyme reactors in HPLC and its application in inhibitor screening:A review
    Determination of drug,excipients and coating distribution in pharmaceutical tablets using NIR-CI
    Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation(Rajeev Jain[1] Ramkishor Sharma[2])
    Validated stability indicating methods for determination of nitazoxanide in presence of its degradation products
    The expert system of genotype discrimination for D5S818 locus based on near-infrared spectroscopy-principal discriminant variate
    Voltammetric behavior of sedative drug midazolam at glassy carbon electrode in solubilized systems(Rajeev Jain[1] Rajeev Kumar Yadav[2])
    Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein
    Development and validation of RP-HPLC and RP-UPLC methods for quantification of parathyroid hormones (1-34) in medicinal product formulated with meta-cresol
    Separation,identification,and quantification of active constituents in Fructus Psoraleae by high-performance liquid chromatography with UV,ion trap mass spectrometry,and electrochemical detection
    ORIGINAL ARTICLE Development and validation of a normal-phase HPTLC method for the simultaneous analysis of Lamivudine and Zidovudine in fixed-dose combination tablets
    New fluorimetric assay of horseradish peroxidase using sesamol as substrate and its application to EIA
    Application of analytical instruments in pharmaceutical analysis
    JPA Prize in 2011
    《药物分析学报:英文版》封面

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