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pepo
  • 西葫芦未受精胚珠离体培养条件的优化及胚囊植株的产生 免费阅读 下载全文 相关:西葫芦(Cucurbita pepo L.)
  • 【目的】建立西葫芦离体雌核发育高频植株再生体系,以加速自交系选育,有效缩短西葫芦杂种一代育种周期。【方法】将西葫芦未受精胚珠接种至附加2,4-D、NAA、BA的N6培养基上可形成胚状体,该胚状体转接至无激素的N6培养基上可形成再生植株。【结果】试验共获得120棵再生植株(R0),其中41株因生活力低下死亡,8株用于摸索移栽方法死亡,71株移栽成活且生长正常,其中42株性状表现符合二倍体特征且育性正常,已有10株获得自交果实及种子,其余29株育性异常。试验结果表明,胚珠发育时期、培养基、供体基因型及供体栽培季节等均显著影响胚状体诱导频率。【结论】胚状体起源于胚囊成员细胞,再生植株为胚囊植株。试验筛选出3种诱导频率较高的培养基;以开花前1日及当日的胚珠诱导频率较高;秋播材料诱导效果最好。
  • 温度和湿度对西葫芦贮藏过程中品质的影响 免费阅读 下载全文 相关:西葫芦(Cucurbita pepo L.)
  • 研究了室温(28-32℃)和10℃条件下不同湿度(低湿、饱和湿度)对西葫芦(Cucurbita pepo L.)采后贮藏品质的影响。结果表明,随着贮藏时间的延长,西葫芦表皮颜色趋于黄化,水分含量下降,萎蔫和腐烂程度加深。低温和饱和湿度处理可以延缓颜色指数、萎蔫指数和腐烂指数的上升以及叶绿素、维生素C和可溶性蛋白含量的下降,减少西葫芦瓜肉和表皮水分的损失,较好地保存西葫芦的外观和营养品质。
  • 蚯蚓粪在西葫芦穴盘育苗中的应用 免费阅读 下载全文 相关:蚯蚓粪 西葫芦(Cucurbita pepo
  • 以蚯蚓粪为主要原料配合不同体积比的珍珠岩作为育苗基质,研究了复合基质对西葫芦(Cuc urb ita pepo L.)种子萌发及幼苗生长发育的影响。结果表明,与草炭相比,蚯蚓粪具有有机质含量高,营养丰富,氮、磷、钾含量高等特性;蚯蚓粪和珍珠岩以3:1(V/V )混合基质育苗效果最佳,既提高了西葫芦种子的出苗率,又促进了幼苗的生长发育,地上部干重、地下部干重、根系活力和壮苗指数分别是对照的1.95、2.20、1.34和2.20倍。
  • 西葫芦穴盘育苗潮汐灌溉技术研究 免费阅读 下载全文 相关:西葫芦(Cucurbita pepo L.)
  • 以西葫芦(Cucurbita pepo L.)为试材,采用潮汐灌溉和顶部洒水(CK)2种灌溉方式,其中潮汐灌溉有2个灌溉时长水平:15 min/次、30 min/次,3个灌溉频率水平:1次/d、1次/2 d、1次/3 d,共7个处理;探讨了潮汐灌溉和顶部洒水2种灌溉方式对西葫芦穴盘苗生长及生理变化、基质电导率、含水量和灌水量的影响。结果表明,与顶部洒水灌溉方式相比,潮汐灌溉的15 min/次、1次/d处理和30 min/次、1次/d处理的穴盘苗素质最佳,且分别节水10%、6%;最优潮汐灌溉制度是灌溉高度为穴盘高度的2/3(3 cm)、灌溉时长为15 min/次、灌溉频率为1次/d。
  • 痕量灌溉技术在日光温室西葫芦栽培上的应用研究 免费阅读 下载全文 相关:痕量灌溉 西葫芦(Cucurbita pepo
  • 以西葫芦(Cucurbita pepo L.)为材料,微喷灌溉为对照,研究痕量灌溉管不同埋深对日光温室春季西葫芦生长、产量和水分生产效率的影响,结果表明,痕量灌溉管不同埋深处理后对西葫芦的生长影响无差异性;与对照相比,埋深6、12 cm 2个处理的西葫芦叶片蒸腾速率分别降低了1.26、1.72 mmol(H2O)/(m^2·s),叶片净光合速率分别提高了1.40、1.09μmol(CO2)/(m^2·s),果实有机酸含量分别降低了17.2%、6.9%;微喷灌溉的果实VC含量分别比2个处理降低了32.7%、30.5%。在0-6 cm深基质里含水量高低表现为微喷灌溉〉痕量灌溉管埋深6 cm〉痕量灌溉管埋深12 cm,在6-12 cm深基质里含水量高低表现为微喷灌溉〉痕量灌溉管埋深12 cm〉痕量灌溉管埋深6 cm;痕量灌溉管埋深6、12 cm的处理其667 m^2的耗水量分别比微喷灌溉下降了65.7%、61.7%,水分生产效率分别增加了102.4%、88.1%。综合产量、耗水量等经济因素,西葫芦基质栽培的痕量管埋深以6 cm为宜。
  • 西葫芦茎尖离体培养和快速繁殖技术 相关:西葫芦(Cucurbita pepo L.)
  • 以西葫芦茎尖为外植体,MS为基本培养基,探索了不同消毒处理、不同浓度的植物生长调节剂及其组合对西葫芦茎尖再生和快速繁殖的影响。结果表明:西葫芦茎尖在含有0.02%升汞与10%次氯酸钠的溶液中消毒10min的效果最好,污染率降低到6.7%,成活率可达91.7%;西葫芦茎尖诱导再生芽的最好培养基处理为MS+6-BA 0.5mg/L+IBA 0.10mg/L;最佳的生根诱导培养基为MS+IBA 0.30mg/L。
  • PepO通过上调腹腔巨噬细胞中MircroRNA-155的表达来抑制IL-6的产生 相关:PepO MircroRNA-155 MYD88
  • 探索microRNA-155在细胞炎症反应调控中的作用机制。采用重组PepO蛋白刺激小鼠腹腔巨噬细胞(PEMs)诱导的microRNA-155(miR-155)及其对IL-6的调节。PepO刺激腹腔巨噬细胞诱导miRNA-155呈剂量和时间依赖性表达;Mimic和inhibitor转染实验显示过表达mi R-155可抑制IL-6及My D88的表达;IL-6及MyD88与PepO也呈剂量依赖性;且IL-6与MyD88存在PepO处理时间的相关性。这些结果提示,PepO刺激腹腔巨噬细胞诱导miRNA-155可能通过靶向myD88通路信号从而抑制IL-6的表达,避免宿主不至于过度炎症反应导致炎症损伤。
  • Fatty Acid Accumulation in Seeds of Three Seed Pumpkin Cultivars 免费阅读 下载全文 相关:SEED PUMPKIN CUCURBITA
  • In the literature there are many reports on the composition and properties of pumpkin seed oil; however, few is known about the effect of different stages of seed development on various fatty acid profiles in developing seeds. The objective of this study was to provide the changes of various fatty acid accumulations in seed oil obtained from the seeds of three pumpkin varieties belonging to the species Cucurbita maxima and Cucurbita pepo. Unsaturated acids (oleic and linoleic) were dominant in various fatty acids, which constituted 38.9%-49.1% and 29.4%-42.7% of the total fatty acids at seed maturity for three pumpkin varieties, respectively, while other fatty acid concentrations except for palmitic acid all did not reach 10%. Different varieties exhibited greater effect on various fatty acid contents and the total fatty acid contents in the seeds of pumpkin rather than the species. On the whole, palmitic acid profiles of the seed oil in three varieties all followed the fluctuant decrease during all the stages of seed development, but palmitoleic acid and the total fatty acid profiles of the seed oil in three varieties were just the opposite. Stearic, oleic and linoleic acid profiles of the seed oil in three varieties all experienced the third pattern that fluctuated during all the stages of seed development, but no significant differences in these three fatty acid concentrations were found from the beginning to the end. Linolenic acid concentrations of three varieties were on the decline and ultimately close to zero. Myristic and arachidic acid profiles of the seed oil followed different trends in three varieties. Among them, myristic and arachidic acid profiles of the seed oil of Yinhui-1 fluctuated downward all the time until seed maturity, but those of 0238-1 and Jinhui-2 completely changed oppositely.

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